BETA GRANULE FORMATION IN ISOLATED ISLETS OF LANGERHANS
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چکیده
منابع مشابه
Beta Granule Formation in Isolated Islets of Langerhans
The distribution of radioautographic grains over organelles within the beta cells of rat islets of Langerhans was investigated at various times after pulse labeling of the isolated islets with tritium-labeled amino acids. Ten minutes after the start of labeling most of the grains were situated over the endoplasmic reticulum and cytoplasm; by contrast, 60 min from the start of labeling the major...
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Isolation of rat islets of Langerhans was carried out by using collagenase and the destruction of exocrine tissue was performed through the injection of Hanks' solution into the common bile duct. The identification of the islets was done by techniques including the use of a stereomicroscope against a black background. Vital staining was done by an injection of neutral red solution through t...
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Incretins such as glucagon-like peptide 1 (GLP-1) are released from the gut and potentiate insulin release in a glucose-dependent manner. Although this action is generally believed to hinge on cAMP and protein kinase A signaling, up-regulated beta cell intermediary metabolism may also play a role in incretin-stimulated insulin secretion. By employing recombinant probes to image ATP dynamically ...
متن کاملPhosphatidylinositol hydrolysis in isolated guinea-pig islets of Langerhans.
Previous studies have reported an increased turnover of phospholipid in isolated islets of Langerhans in response to raised glucose concentrations. The present investigation was thus undertaken to determine the nature of any phospholipases that may be implicated in this phenomenon by employing various radiolabelled exogenous phospholipids. Hydrolysis of 1-acyl-2-[14C]arachidonoylglycerophosphoi...
متن کاملISOLATION AND PROPERTIES OF SECRETORY GRANULES FROM RAT ISLETS OF LANGERHANS II. Ultrastructure of the Beta Granule
Beta granules isolated from rat islets of Langerhans and subjected only to phosphotungstic acid had, in negatively stained images, a 50-A periodicity . This periodicity was also observed in thin-section profiles of beta granules in intact cells . In shadowed preparations, the granules were spherical in shape and had irregular edges and surface structure . The presence of such a periodicity in t...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1969
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.42.3.695